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Culture of Animal Cells : A Manual of Basic Technique and Specialized Applications by Freshney R and R. Ian Freshney (2010, Hardcover)
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Freshney's Culture of Animal Cells : A Manual of Basic Technique 6th Edition ISBN978-0-470-52812-9.
- Buy It NowFreshney's Culture of Animal Cells : A Manual of Basic Technique 6th Edition
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About this product
Product Identifiers
PublisherWiley & Sons, Incorporated, John
ISBN-100470528125
ISBN-139780470528129
eBay Product ID (ePID)84251134
Product Key Features
Number of Pages768 Pages
Publication NameCulture of Animal Cells : a Manual of Basic Technique and Specialized Applications
LanguageEnglish
Publication Year2010
SubjectLife Sciences / Cell Biology
TypeTextbook
AuthorFreshney R, R. Ian Freshney
Subject AreaScience
FormatHardcover
Dimensions
Item Height1.8 in
Item Weight0.1 Oz
Item Length11.3 in
Item Width8.8 in
Additional Product Features
Edition Number6
Intended AudienceScholarly & Professional
LCCN2010-007042
Dewey Edition22
Reviews"The book's sixth incarnation includes new sections on organotypic culture and different cell and stem cell types". (Veterinary Times, 11 April 2011)
IllustratedYes
Dewey Decimal571.5381
SynopsisThe most complete resource on the techniques, equipment, principles, and practices of animal cell culture Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded Sixth Edition of Culture of Animal Cells , the leading text in the basic methodology of cell culture. This authoritative volume offers a complete background related to growth of animal cells in culture. It contains many specialized protocols and advice on how to apply some of the basic protocols to teaching cell culture. Beginning with laboratory design, safety, validation, and bioethics, then continuing with preparation of media, primary culture, and cell lines through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the unparalleled coverage includes: An all-new section addressing the additional caution required once a primary culture is converted to a cell line Differentiation in stem cells and reprogramming of adult cells to form stem cells A new section and protocol on cryopreservation by vitrification Separate sections dealing with epithelial cells, mesenchymal cells, neurectodermal cells, and hematopoietic cells, a new chapter on germs cells/stemcells/amniocytes and non-mammalian/avain cells (other vertebrates and invertebrates) A new section on organotypic culture Increased emphasis on contamination and misidentification (including cross-contamination) with revised protocols for detection and elimination A problem-solving chapter, glossary of terms, advice on sourcing materials, and list of suppliers This landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells and is indispensable for those in the field, including clinical and biopharmaceutical researchers and scientists, graduate students, laboratory managers in cell and molecular biology labs and genetics labs, trainees, and technicians., This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer., This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs. PowerPoint slides of the figures as well as other supplementary materials are available at a companion website: www.wiley.com/go/freshney/cellculture, This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs. PowerPoint slides of the figures as well as other supplementary materials are available at a companion website: www.wiley.com/go/freshney/cellculture
LC Classification NumberQH585.2.F74 2010
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