Picture 1 of 8
Stock photo
Picture 1 of 8
Stock photo
Methods in Molecular Biology Ser.: HPLC of Peptides and Proteins : Methods and Protocols by Marie Isabel Aguilar (2003, Hardcover)
V
vaindioux (2887)
100% positive feedback
Price:
$99.00
+ $5.97 shipping
Returns:
30 days returns. Seller pays for return shipping.
Condition:
This textbook is a comprehensive guide for those interested in the subject areas of science and medical immunology. The author, Marie Isabel Aguilar, provides a detailed discussion of the methods and protocols involved in HPLC analysis of peptides and proteins. The book covers a range of topics, including the life sciences and biochemistry, and is published by Humana Press under the Methods in Molecular Biology Ser. series. The book measures 9 inches in length and 6.1 inches in width, with a weight of 60.7 ounces. It contains a total of 414 pages, including an introduction and detailed discussions of the various techniques and protocols involved in HPLC analysis. This textbook is an essential resource for anyone interested in the subject area of immunology and medical research.
- Buy It NowHPLC of Peptides and Proteins: Methods and Protocols, Aguilar-, Brand New
Oops! Looks like we're having trouble connecting to our server.
Refresh your browser window to try again.
About this product
Product Identifiers
PublisherHumana Press
ISBN-100896039773
ISBN-139780896039773
eBay Product ID (ePID)2297768
Product Key Features
Number of PagesXiv, 414 Pages
LanguageEnglish
Publication NameHplc of Peptides and Proteins : Methods and Protocols
Publication Year2003
SubjectImmunology, Life Sciences / Biochemistry
TypeTextbook
Subject AreaScience, Medical
AuthorMarie Isabel Aguilar
SeriesMethods in Molecular Biology Ser.
FormatHardcover
Dimensions
Item Weight60.7 Oz
Item Length9 in
Item Width6.1 in
Additional Product Features
Intended AudienceScholarly & Professional
LCCN2003-017464
Dewey Edition22
Reviews"...a showcase of state-of-the-art methodologies in the application of HPLC to protein/peptide studies...can be used by beginners and intermediate experts in this field to develop separation and analytical protocols...all scientists dealing with peptides and proteins can benefit from reading this book." - Proteomics "...this book is well written...a very usable laboratory companion or reference guide for researchers in pursuit of peptide and protein characterizations." - Biomedical Chromatography
Series Volume Number251
Number of Volumes1 vol.
IllustratedYes
Dewey Decimal572/.6536
Table Of ContentModes of HPLC.- HPLC of Peptides and Proteins.- Reversed-Phase High-Performance Liquid Chromatography.- Ion-Exchange Chromatography.- High-Performance Hydrophobic Interaction Chromatography.- Gel Filtration Chromatography.- Hydrophilic Interaction Chromatography.- Immobilized Metal Ion Affinity Chromatography of Proteins.- Immunoaffinity Chromatography of Proteins.- Liquid Chromatography-Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteins.- Preparative Methodologies.- Capillary Separations.- Micropreparative HPLC of Peptides and Proteins.- Multidimensional HPLC Purification of Proteins.- Analytical High-Performance Liquid Chromatography.- Prep/Semiprep Separations of Peptides.- Large-Scale Protein Chromatography.- Applications.- Proteolytic Peptide Mapping.- Mass Spectrometric Characterization of Posttranslationally Modified Proteins--Phosphorylation.- Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography-Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry.- HPLC in the Analysis of Peptide Metabolism.- Isolation and Characterization of Naturally Processed MHC-Bound Peptides From the Surface of Antigen-Presenting Cells.- Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques.- HPLC and Mass Spectrometry of Intrinsic Membrane Proteins.- IgG Purification.- DNA-Binding Proteins.- Sensitive Enzymatic Analysis of Histidine Decarboxylase Using HPLC.- Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry.- Proteome Analysis.
SynopsisThis text provides the novice with the practical information needed to begin developing separation and analytical protocols for peptide and protein analysis. The author leads in with an overview of the basic theory and general methodology of HPLC, with particular reference to the key separation parameters, which can be manipulated to achieve high resolution. Each of the most commonly used HPLC techniques are covered in the following chapters in addition to a number of case studies as examples. These examples help illustrate the analytical approaches to a particular separation or assay challenge with examples drawn from contemporary fields in biochemistry and biotechnology. Example applications include proteolytic mapping, post-membrane proteins and lipoproteins. Further chapters give concise coverage to combinatorial analysis and enzymatic activity. In closing chapters, the text covers HPLC as a physicochemical tool for studying peptide and protein folding. This text is an invaluable tool for scientists working with peptides and or proteins in both academic and biotechnology laboratories., The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.
LC Classification NumberQD415-436
