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About this product
Product Identifiers
PublisherSpringer New York
ISBN-101493925466
ISBN-139781493925469
eBay Product ID (ePID)208627507
Product Key Features
Number of PagesXi, 238 Pages
LanguageEnglish
Publication NameSmall Non-Coding Rnas : Methods and Protocols
Publication Year2015
SubjectLife Sciences / Biochemistry, Genetics
TypeTextbook
Subject AreaScience, Medical
AuthorMathieu Rederstorff
SeriesMethods in Molecular Biology Ser.
FormatHardcover
Dimensions
Item Weight239.4 Oz
Item Length10 in
Item Width7 in
Additional Product Features
Intended AudienceScholarly & Professional
LCCN2015-934152
Dewey Edition23
Series Volume Number1296
Number of Volumes1 vol.
IllustratedYes
Dewey Decimal572.8/8
Table Of ContentSmall Non-Coding RNAs: A Quick Look in the Rear-View Mirror.- Alcoholic Precipitation of Small Non-Coding RNAs.- Quantification and Quality Control of a Small Non-Coding RNA Preparation.- Impact of RNA Isolation Protocols on RNA Detection by Northern Blotting.- Improved Northern Blot Detection of Small RNAs Using EDC Crosslinking and DNA/LNA Probes.- Direct Cloning of Double Stranded RNAs.- Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation.- Fluorescence in situ Hybridization of Small Non-Coding RNAs.- RT-qPCR Based Quantification of Small Non-Coding RNAs.- Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAs.- miR-RACE: An Effective Approach to Accurately Determine the Sequence of Computationally Identified miRNAs.- Probing Small Non-Coding RNAs Structures.- cDNA Library Generation for the Analysis of Small RNAs by High Throughput Sequencing.- CLIP-seq to Discover Transcriptome-Wide Imprinting of RNA Binding Proteins in Living Cells.- Microarray Analysis of Small Non-Coding RNAs.- RLM-RACE, PPM-RACE and qRT-PCR, an Integrated Strategy to Accurately Validate miRNA Target Genes.- Dual Luciferase Gene Reporter Assays to Study miRNA Function.- Gene Expression Knockdown by Transfection of siRNAs into Mammalian Cells.- Efficient and Selective Knockdown of Small Non-Coding RNAs.- Cell-SELEX: In Vitro Selection of Synthetic Small Specific Ligands.- Small Non-Coding RNAs and Aptamers in Diagnostics and Therapeutics.
SynopsisThis volume contains state-of-the-art methods tackling all aspects of small non-coding RNAs biology. Small Non-Coding RNAs: Methods and Protocols guides readers through customized dedicated protocols and technologies that will be of valuable help to all those willing to contribute deciphering the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols reaches out to biochemists, cellular and molecular biologists already working in the field of RNA biology and to those just starting to study small non-coding RNAs.
LC Classification NumberRB155-155.8
