About this product

Product Identifiers

PublisherElsevier Science & Technology

ISBN-100123813778

ISBN-139780123813770

eBay Product ID (ePID)79670202

Product Key Features

Number of Pages416 Pages

LanguageEnglish

Publication NameCilia: Model Organisms and Intraflagellar Transport

SubjectLife Sciences / Cell Biology, Life Sciences / Biophysics

Publication Year2009

TypeTextbook

AuthorGregory J. Pazour

Subject AreaScience

SeriesMethods in Cell Biology Ser.

FormatHardcover

Dimensions

Item Length9.2 in

Item Width7.5 in

Additional Product Features

Edition Number93

Intended AudienceScholarly & Professional

Dewey Edition22

Series Volume NumberVolume 93

IllustratedYes

Volume NumberVolume 93

Dewey Decimal571.67

Table Of Content1. Manipulating Ciliary Protein-Encoding Genes in Tetrahymena thermophila Drashti Dave, Dorota Wloga, and Jacek Gaertig 2. Approaches for Functional Analysis of Flagellar Proteins in African Trypanosomes Michael Oberholzer, Miguel A. Lopez, Katherine S. Ralston, and Kent L. Hill 3. Tools for Analyzing Intraflagellar Transport in Trypanosomes Daria Julkowska and Philippe Bastin 4. Schmidtea mediterranea: A Model System for Analysis of Motile Cilia Panteleimon Rompolas, Ramila S. Patel-King, and Stephen M. King 5. Targeted Gene Silencing by RNA Interference in Chlamydomonas Eun-Jeong Kim and Heriberto Cerutti 6. Analysis of Cargo Transport by IFT and GFP Imaging of IFT in Chlamydomonas Dennis Diener 7. Genetic and Phenotypic Analysis of Flagellar Assembly Mutants in Chlamydomonas reinhardtii Carlo Iomini, Jacob E. Till, and Susan K. Dutcher 8. Recording and Analyzing IFT in Chlamydomonas Flagella William Dentler, Kristyn VanderWaal, and Mary E Porter 9. Total Internal Reflection Fluorescence (TIRF) Microscopy of Chlamydomonas Flagella Benjamin D. Engel, Karl-Ferdinand Lechtreck, Tsuyoshi Sakai, Mitsuo Ikebe, George B. Witman, and Wallace F. Marshall 10. Purification of IFT Particle Proteins and Preparation of Recombinant Proteins for Structural and Functional Analysis Robert H. Behal, Ewelina Betleja, and Douglas G. Cole 11. Studying Cilia in Zebrafish Iain Drummond 12. Analysis of IFT Kinesins in Developing Zebrafish Cone Photoreceptor Sensory Cilia Christine Insinna, Katherine Luby-Phelps, Brian A. Link, and Joseph C. Besharse 13. Analysis of Intraflagellar Transport in C. elegans Sensory Cilia Limin Hao, Seyda Acar, James Evans, Guangshuo Ou, and Jonathan M. Scholey 14. Functional Genomics of Intraflagellar Transport-Associated Proteins in C. elegans Peter N. Inglis, Oliver E. Blacque, and Michel R. Leroux 15. Generating Conditional Mutants to Analyze Ciliary Functions: The Use of Cre-Lox Technology to Disrupt Cilia in Specific Organs Amber K. O'Connor, Robert A. Kesterson, and Bradley K. Yoder 16. Imaging Intraflagellar Transport in Mammalian Primary Cilia Tatiana Y. Besschetnova, Barnali Roy, and Jagesh V. Shah 17. Analysis of Hedgehog Signaling in Mouse Intraflagellar Transport Mutants Hyuk W. Ko, Aimin Liu, and Jonathan T. Eggenschwiler

SynopsisCilia are highly conserved organelles that serve motile functions, sensory functions, or both. These organelles power cell movement, generate fluid flow in various organs, act as sensors of the extracellular environment and have been modified for various specialized tasks such as light reception and smell. Defects in these ubiquitous organelles lead to a broad array of human genetic disorders that range from polycystic kidney disease, retinal degeneration, epilepsy and infertility to developmental defects such as situs inversus and polydactyly. This volume is the third in a three-part series on cilia that focuses on the use of model organisms to gain insight into ciliary function and on the process of intraflagellar transport that is essential for the assembly and maintenance of ciliary structures., Cilia are highly conserved organelles that serve motile functions, sensory functions, or both. These organelles power cell movement, generate fluid flow in various organs, act as sensors of the extracellular environment and have been modified for various specialized tasks such as light reception and smell. Defects in these ubiquitous organelles lead to a broad array of human genetic disorders that range from polycystic kidney disease, retinal degeneration, epilepsy and infertility to developmental defects such as situs inversus and polydactyly. This volume is the third in a three-part series on cilia that focuses on the use of model organisms to gain insight into ciliary function and on the process of intraflagellar transport that is essential for the assembly and maintenance of ciliary structures. * Includes both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Covers forward and reverse genetic analysis of IFT and biochemical methods to define the role of IFT components * Methods presented cover molecular, genetic, and biochemical approaches to ciliary function in model organisms