The item you selected is unavailable, but we found something similar.

Unraveling DNA: Molecular Biology for the Laboratory by Winfrey, Michael, Rott, - Picture 1 of 1

Unraveling DNA : Molecular Biology for the Laboratory by Marc Rott, Michael Winfrey and Alan Wortman (1997, Trade Paperback)

4.01 product rating

textbooks_source (32059)
99.1% positive feedback

Price:

$99.99

Free shipping

Estimated delivery Wed, May 15 - Sat, May 18

Returns:

30 days returns. Buyer pays for return shipping.

Condition:

Brand New

Unraveling DNA: Molecular Biology for the Laboratory. Title : Unraveling DNA: Molecular Biology for the Laboratory.

About this product

Product Information

This manual encompasses an integrated series of molecular biology laboratory exercises that involve the cloning and analysis of the bioluminescence (lux) genes from the marine bacterium Vibrio fischeri. KEY TOPICS: The manual is divided into discrete units with each demonstrating one or more aspects of the cloning project. The manual is based on one of nature's most fascinating biological phenomenon: the biological production of light. This results in a recurrent theme of interest and makes the project very relevant to interdisciplinary topics such as fish symbiosis, biochemistry, biophysics, etc. Includes instruction in the basic techniques of modern molecular biology: DNA isolation and analysis, DNA restriction, agarose gel electrophoresis, ligations, transformation of recombinant DNA, preparation and screening a genomic library, restriction mapping, Southern blotting, hybridization, DNA sequencing, pulsed field gel electrophoresis. MARKET: Designed for a one semester course in Molecular Biology. Also appropriate for a molecular biology component of Microbial Genetics, Genetics, Biochemistry, or Advanced Microbiology courses.

Product Identifiers

Publisher
Pearson Education
ISBN-10
0132700344
ISBN-13
9780132700344
eBay Product ID (ePID)
652193

Product Key Features

Author
Alan Wortman, Michael Winfrey, Marc Rott
Publication Name
Unraveling D N A : Molecular Biology for the Laboratory
Format
Trade Paperback
Language
English
Publication Year
1997
Type
Textbook
Number of Pages
400 Pages

Dimensions

Item Length
10.8in
Item Height
1in
Item Width
8.1in
Item Weight
31.2 Oz

Additional Product Features

Lc Classification Number
Qh506.W54 1997
Edition Number
1
Table of Content
I. INTRODUCTORY TECHNIQUES. 1. Introduction to the Laboratory: Basic Equipment and Bacteriological Techniques. 2. Preparation of Media and Reagents Used in Molecular Biology. 3. Isolation of Luminescent Bacteria from Natural Sources. 4. Restriction Digestion and Agarose Gel Electrophoresis of DNA. II. DNA ISOLATION AND ANALYSIS. 5. Isolation of Chromosomal DNA from Vibrio fischeri. 6. Large-Scale Purification of Plasmid DNA. 7. Spectrophotometric Analysis of DNA. III. CLONING THE LUX OPERON 8. Restriction Digestion of Vibrio fischeri Genomic DNA and Plasmid Vector. 9. Quantification of Genomic DNA by Fluorometry and Agarose Plate Fluorescence. 10. Ligation of Restriction Fragments of Vibrio fischeri DNA to Plasmid Vector. 11. Preparation of Competent Escherichia coli DH5. 12. Transformation of Competent Escherichia coli DH5 with Recombinant Plasmids. 13. Screening the Vibrio fischeri Genomic Library for Light Producing Clones. IV. RESTRICTION MAPPING AND SOUTHERN BLOTTING 14. Small-Scale Plasmid Isolations (Mini-preps) from Bioluminescent Clones. 15. Restriction Mapping of Plasmids from Bioluminescent Clones. 16. Southern Blotting and Hybridization to Detect the luxA Gene. V. SUBCLONING THE LUX A GENE 17. Restriction Digestion of lux Plasmids and Cloning Vector for Subcloning luxA. 18. Gel Purification of DNA Restriction Fragments Containing luxA. 19. Subcloning luxA into A Plasmid Vector. 20. Transformation of Competent Escherichia coli DH5 with Subcloned DNA. 21. Colony Hybridization to Screen for luxA Subclones. 22. Small-Scale Plasmid Isolations (Mini-Preps) from luxA Clones. VI. ADVANCED TECHNIQUES. 23. Amplification of luxA from Natural Isolates by the Polymerase Chain Reaction (PCR). 24. Southern Blotting and Hybridization of PCR Products. 25. DNA Sequencing of lux Genes from Plasmid Templates. 26. Computer Analysis of DNA Sequences Using the World Wide Web. 27. Mapping the Vibrio fischeri Genome by Pulsed Field Gel Electrophoresis. 28. Independent Projects in Molecular Biology. APPENDICES. 1. The Metric System and Units of Measure. 2. Centrifugation. 3. Spectrophotometry. 4. Agarose and Polyacrylamide Gel Electrophoresis. 5. Methylene Blue Staining of Agarose Gels. 6. Nucleic Acid Hybridization. 7. Alcohol Precipitation of Nucleic Acids. 8. Care and Handling of Enzymes. 9. Restriction Endonucleases. 10. Enzymes Used in Molecular Biology. 11. Maps of Cloning Vectors and Bacteriophage Lambda. 12. Proper Handling and Disposal of Hazardous Materials. 13. Procedures and Precautions for the Use of Radioisotopes. 14. Equipment and Supplies. 15. Media and Reagents. 16. Bacterial Strains. 17. Lists of Suppliers. 18. Recommended References. 19. Restriction Mapping Problems.
Copyright Date
1997
Topic
Life Sciences / Molecular Biology, Life Sciences / Biochemistry
Lccn
96-036610
Dewey Decimal
574.87/3282
Intended Audience
College Audience
Dewey Edition
20
Illustrated
Yes
Genre
Science

All listings for this product

item 1 Unraveling DNA: Molecular Biology for the Laboratory
$5.67
Free shipping
item 2 Unraveling DNA: Molecular Biology for the Laboratory
$6.06
Last oneFree shipping
item 3 Unraveling DNA: Molecular Biology for the Laboratory - Paperback - GOOD
$8.44
Free shipping
item 4 Unraveling DNA: Molecular Biology for..., Wortman, Alan
$8.69
Free shipping
item 5 Unraveling DNA : Molecular Biology for the Laboratory Paperback
$12.43
Free shipping
item 6 UNRAVELING DNA: MOLECULAR BIOLOGY FOR THE LABORATORY By Michael R. Mint
$40.95
Free shipping
item 7 Unraveling DNA: Molecular Biology for the Laboratory by Winfrey, Michael, Rott,
$99.99
Free shipping

See all 9

4.0

4.0 out of 5 stars based on 1 product rating

1 product rating

0 users rated this 5 out of 5 stars
1 users rated this 4 out of 5 stars
0 users rated this 3 out of 5 stars
0 users rated this 2 out of 5 stars
0 users rated this 1 out of 5 stars

Most relevant reviews

4 out of 5 stars
by Z3rxxWfOTVK@Del...Oct 17, 2006
Unraveling DNA Lab Textbook
This book is really good in technique but when used in class some of the procedures seemed to be lacking and did not follow the order the sequence of the lab class itself. Had to buy for a class.

Best Selling in Textbooks

See all

Current slide {CURRENT_SLIDE} of {TOTAL_SLIDES}- Best Selling in Textbooks

Diagnostic and Statistical Manual of Mental Disorders DSM-5-TR by American Psychiatric Association (2022, Trade Paperback)
4.6 out of 5 stars based on 15 product ratings
$35.70 New
$32.99 Used
Self Heal by Design : The Role of Micro-organisms for Health by Barbara O'Neill (2018, Trade Paperback)
5.0 out of 5 stars based on 14 product ratings
$29.99 New
---- Used
Leadership : Theory and Practice by Peter G. Northouse (2021, Trade Paperback, Revised edition)
5.0 out of 5 stars based on 1 product rating
$19.98 New
$10.00 Used
Research Design : Qualitative, Quantitative, and Mixed Methods Approaches by J. David Creswell and John W. Creswell (2022, Trade Paperback)
4.2 out of 5 stars based on 5 product ratings
$20.98 New
$18.00 Used
Designing Data-Intensive Applications : The Big Ideas Behind Reliable, Scalable, and Maintainable Systems by Martin Kleppmann (2017, Trade Paperback)
4.9 out of 5 stars based on 17 product ratings
$18.24 New
$15.86 Used
Cognitive Behavior Therapy : Basics and Beyond by Judith S. Beck (2020, Hardcover)
5.0 out of 5 stars based on 4 product ratings
$19.80 New
$17.66 Used
Encyclopedia of Counseling : Master Review and Tutorial for the National Counselor Examination, State Counseling Exams, and the Counselor Preparation Comprehensive Examination by Howard Rosenthal (2017, Trade Paperback, Revised edition,New Edition)
4.9 out of 5 stars based on 17 product ratings
$22.99 New
$21.90 Used

Save on Textbooks

Current slide {CURRENT_SLIDE} of {TOTAL_SLIDES}- Save on Textbooks

Diagnostic and Statistical Manual of Mental Disorders DSM-5-TR Brand new
$32.00
Trending at $42.48
International Building Code IBC 2021 International Code Council Paperback
$49.99
Trending at $63.99
International Code Council Ser.: 2021 International Residential
$52.49
Trending at $68.00
National Electrical Code Handbook, 2023 Edition
$62.99
Trending at $69.00
National Electrical Code (NEC) NFPA 70 2020 Edition Spiral USA STOCK
$33.90
Trending at $36.40
Publication Manual of the American Psychological Association: 7th Edition, 2020
$14.45
Trending at $15.99