About this product

Product Identifiers

PublisherElsevier Science & Technology

ISBN-100123749735

ISBN-139780123749734

eBay Product ID (ePID)77198460

Product Key Features

Number of Pages344 Pages

LanguageEnglish

Publication NameCilia: Structure and Motility

SubjectLife Sciences / Cell Biology, Life Sciences / Anatomy & Physiology (See Also Life Sciences / Human Anatomy & Physiology)

Publication Year2009

TypeTextbook

Subject AreaScience

AuthorGregory J. Pazour

SeriesMethods in Cell Biology Ser.

FormatHardcover

Dimensions

Item Length10.2 in

Item Width7.2 in

Additional Product Features

Edition Number91

Intended AudienceScholarly & Professional

Dewey Edition22

Series Volume NumberVolume 91

Volume NumberVolume 91

IllustratedYes

Dewey Decimal571.67

Table Of Content1. RNAi approaches to axonemal motor function in trypanosomes (Hill) 2. Homologous recombination to knock out dynein genes in Tetrahymena (Gaertig) 3. RNAi in Chlamydomonas (Cerutti) 4. Planarians as a model system for analysis of ciliary assembly/motility (Rompolas) 5. Role of nucleotides in dynein function (Shingyoji) 6. Protein modification to probe dynein interactions + purification and identification of crosslinked dynein products (Sakato) 7. Analysis of redox-sensitive dynein components (Wakabayashi) 8. Purification of dyneins from Chlamydomonas and analysis of dynein-dynein linkers (Kamiya/King) 9. Kinase/phosphatase-mediated control of dynein function (Sale) 10. Central pair MT complex and associated kinesins (Mitchell) 11. Calcium regulation of axonemal function In vitro motility assays (Smith) 12. Identification and analysis of dynein regulatory complex components (Porter) 13. Isolation and analysis of radial spoke proteins (Yang) 14. Purification of dyneins from other model organisms: Ciona, sea urchin, fish (Inaba) 15. Rescue of mutant phenotypes by protein electroporation (Kamiya Lab) 16. Tubulin/Dynein interactions (Raff) 17. Measurement of beat frequency and swimming velocity Cell model reactivation in vitro assays of dynein function (Kamiya) 18. Live imaging of ependymal cilia (Lechtreck/Witman) 19. Immunogold labeling of axonemal components in situ + flat embedding (Geimer) 20. CryoEM approaches to axonemal organization (Nicastro) 21. CryoEM of dynein-microtubule complexes (Oda/Kikkawa) 22. Bioinformatic approaches to dynein HC classification (Yagi) 23. Bioinformatics of non-motor dynein components (Asai) 24. Biophysical approaches to dynein mechanism (Sutoh) 25. Chlamydomonas flagellar beat analysis (Foster) 26. Biophysical measurements of motor function (step size/force production) SAXS of axonemes (Toba/Oiwa) Cryo-Electron Microscope Tomography to Study Axonemal Organization Daniela Nicastro 2. Electron Microscopic Imaging and Analysis of Isolated Dynein Particles Anthony J. Roberts and Stan A. Burgess 3. Immunogold Labeling of Flagellar Components In Situ Stefan Geimer 4. Scanning Electron Microscopy to Examine Cells and Organs Jovenal T. SanAgustin, John A. Follit, Gregory Hendricks, and Gregory J. Pazour 5. X-ray Fiber Diffraction Studies on Flagellar Axonemes Kazuhiro Oiwa, Shinji Kamimura, and Hiroyuki Iwamoto 6. Markers for Neuronal Cilia Jacqueline S. Domire and Kirk Mykytyn 7. Immunofluorescence Staining of Ciliated Respiratory Epithelial Cells Heymut Omran and Niki T. Loges 8. Immunoprecipitation to Examine Protein Complexes Gregory J. Pazour 9. Tandem Affinity Purification of Ciliopathy-Associated Protein Complexes Karsten Boldt, Jeroen van Reeuwijk, Christian Johannes Gloeckner, Marius Ueffing, and Ronald Roepman 10. Crosslinking Methods: Purification and Analysis of Crosslinked Dynein Products Miho Sakato 11. Analysis of the Ciliary/Flagellar Beating of Chlamydomonas Kenneth W. Foster 12. Assays of Cell and Axonemal Motility in Chlamydomonas reinhardtii Ritsu Kamiya 13. High-Speed Digital Imaging of Ependymal Cilia in the Murine Brain Karl-Ferdinand Lechtreck, Michael J. Sanderson, and George B. Witman 14. Observation of Nodal Cilia Movement and Measurement of Nodal Flow Yasushi Okada and Nobutaka Hirokawa 15. Modification of Mouse Nodal Flow by Applying Artificial Flow Shigenori Nonaka 16. Measuring Cilium-Induced Ca2þ Increases in Cultured Renal Epithelia Helle A. Praetorius

SynopsisAlong with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella., Along with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella. * Covers protocols for cilia and flagella across systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures