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Methods in Molecular Biology Ser.: Protein Misfolding and Disease : Principles and Protocols by Niels Gregersen (2003, Hardcover)

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It is now recognized that perturbations of the protein folding process and disturbances of their conformation maintenance are major disease mechanisms. This development has given rise to the concept of conformational diseases as well as to the broader signature of protein folding diseases, comprised of diseases in which mutations or environmental stresses may result in a partial misfolding that then leads to alternative conformations capable of disturbing cellular processes. This book provides a remarkable set of analytical tools that can be used to uncover the characteristics of conformational diseases in patient material or cellular model systems. The protocols can be adapted for researching many different proteins and will be of immense value for scientists studying conformational diseases.

Product Identifiers

Publisher
Humana Press
ISBN-10
1588290654
ISBN-13
9781588290656
eBay Product ID (ePID)
2332430

Product Key Features

Author
Niels Gregersen
Publication Name
Protein Misfolding and Disease : Principles and Protocols
Format
Hardcover
Language
English
Publication Year
2003
Series
Methods in Molecular Biology Ser.
Type
Textbook
Number of Pages
Xv, 318 Pages

Dimensions

Item Length
9.3in
Item Width
6.1in
Item Weight
50.8 Oz

Additional Product Features

Series Volume Number
232
Number of Volumes
1 Vol.
Lc Classification Number
Qd415-436
Reviews
"...a very sound book that provides a solid background in conformational disease and presents the reader with helpful experimental protocols for studying protein folding folding and misfolding." - Pharmaceutical Research "...this book will be a very useful took for specialists in protein chemistry, molecular biology and basic medicine, and also for teachers and their students studying principles of proteomics." Biochemistry
Table of Content
General Concepts and Models.- Protein Misfolding, Aggregation, and Degradation in Disease.- Basic Introduction to In Vivo Protein Folding and Its Defects.- Cystic Fibrosis.- ?1-Antitrypsin Deficiency.- Parkinson's Disease.- Aberrant Protein Folding as the Molecular Basis of Cancer.- Yeast as a Model System to Investigate Protein Conformational Diseases.- General Methods.- Expression of Recombinant Proteins.- Expression in E. coli Systems.- Protein Expression in Yeasts.- Site-Directed Mutagenesis.- Pulse-Chase Labeling Techniques for the Analysis of Protein Maturation and Degradation.- Techniques in Conformational Disease Research.- Detection of Aggregates and Protein Inclusions by Staining of Tissues.- Study of Mutant Proteins With Folding Defects in Cultured Patient Cells.- E. coli Expression System for Identifying Folding Mutations of Human Adenosine Deaminase.- Characterization of Overexpressed Mutant Proteins in Mammalian Cells.- Investigation of Unfolded-Protein Response in Cells Expressing Familial Alzheimer's Disease-Linked Presenilin Variants.- Huntingtin Fragments Form Aggresome-Like Inclusion Bodies in Mammalian Cells.- Application of Chemical Chaperones to the Rescue of Folding Defects.- Analysis of Defective Subunit Interactions Using the Two-Hybrid System.- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in the Cytosol.- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in Microsomes.- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in Mitochondria.- Investigation of Folding and Degradation of Mutant Proteins Synthesized in Semipermeabilized Cells.
Copyright Date
2003
Topic
Life Sciences / Biochemistry, Chemistry / Organic
Lccn
2002-192191
Dewey Decimal
611/.01815
Intended Audience
Scholarly & Professional
Dewey Edition
21
Illustrated
Yes
Genre
Science

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item 1 Protein Misfolding and Disease by Peter Bross: New
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item 2 Protein Misfolding and Disease: Principles and Protocols by Peter Bross (English
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item 3 Protein Misfolding and Disease: Principles and , Zeveloff, Bross, Gregersen-,
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Picture 1 of 1

Methods in Molecular Biology Ser.: Protein Misfolding and Disease : Principles and Protocols by Niels Gregersen (2003, Hardcover)

About this product

Product Information

Product Identifiers

Product Key Features

Dimensions

Additional Product Features

All listings for this product

item 1 Protein Misfolding and Disease by Peter Bross: New

item 2 Protein Misfolding and Disease: Principles and Protocols by Peter Bross (English

item 3 Protein Misfolding and Disease: Principles and , Zeveloff, Bross, Gregersen-,

Best Selling in Textbooks

Desk Reference to the Diagnostic Criteria from DSM-5-TR by American Psychiatric Association (2022, Trade Paperback)

PMBOK® Guide Ser.: Guide to the Project Management Body of Knowledge (PMBOK Guide) and the Standard for Project Management by Project Management Institute (2021, Trade Paperback)

Self Heal by Design : The Role of Micro-organisms for Health by Barbara O'Neill (2018, Trade Paperback)

Diagnostic and Statistical Manual of Mental Disorders DSM-5-TR by American Psychiatric Association (2022, Trade Paperback)

Cognitive Behavior Therapy : Basics and Beyond by Judith S. Beck (2020, Hardcover)

NFPA 70® Handbook with Tabs : 2023 Edition by National Fire Protection Association (NFPA) (2022, Hardcover)

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Save on Textbooks

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